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pbluescript ii sk (+) vector  (Agilent technologies)


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    Structured Review

    Agilent technologies pbluescript ii sk (+) vector
    Pbluescript Ii Sk (+) Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript+ii-sk+vector/bio_rxiv__2023__09__25__559321-31-10-15
    Average 90 stars, based on 1 article reviews
    pbluescript ii sk (+) vector - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Luciferase:

    Article Title: The Polypyrimidine Tract-binding Protein Is Required for Efficient Dengue Virus Propagation and Associates with the Viral Replication Machinery
    Article Snippet: The firefly luciferase genewas amplified from the pGL3Basic vector (Promega) using the primers FlucBamF and FlucNotR and cloned into the pBluescript II-SK vector (Stratagene) at the BamHI and NotI sites (pBS-Fluc).

    Article Title: Corticotropin-releasing factor and its receptors in the brain of rats with insulin and corticosterone deficits.
    Article Snippet: The CRF1R cRNA probe was generated from a 1$3 kb PstI–PstI fragment of the rat prCRF PP1.3-BS cDNA (Dr Vale W, Peptide Biology Laboratory, The Salk Institute) subcloned into a pBluescript II-SK vector (Stratagene) and linearized with BamHI and HindIII to generate antisense and sense probes respectively.

    Article Title: Complex Seizure Disorder Caused by Brunol4 Deficiency in Mice
    Article Snippet: The StuI-digested fragment of ∼3 kb was cloned into the pBluescript II-SK vector (Stratagene, http://www.stratagene.com ).

    Article Title: A single postnatal injection of oxytocin rescues the lethal feeding behaviour in mouse newborns deficient for the imprinted Magel2 gene.
    Article Snippet: The 2.651 kb XhoI and 3.062 kb NotI fragments localized, respectively, 372 and 3062 bp 5′ and 3′ to the transcriptional initiation site of the NT-039428.7 Magel2 mRNA (an intronless gene) were inserted on each side of a LoxP-pgk1-hygromycin-LoxP cassette subcloned into a pBluescript II-SK vector (Stratagene, Agilent, France).

    Article Title: The Polypyrimidine Tract-binding Protein Is Required for Efficient Dengue Virus Propagation and Associates with the Viral Replication Machinery
    Article Snippet: The firefly luciferase gene was amplified from the pGL3-Basic vector (Promega) using the primers Fluc Bam F and Fluc Not R and cloned into the pBluescript II-SK vector (Stratagene) at the BamHI and NotI sites (pBS-Fluc).

    Amplification:

    Article Title: The Polypyrimidine Tract-binding Protein Is Required for Efficient Dengue Virus Propagation and Associates with the Viral Replication Machinery
    Article Snippet: The firefly luciferase genewas amplified from the pGL3Basic vector (Promega) using the primers FlucBamF and FlucNotR and cloned into the pBluescript II-SK vector (Stratagene) at the BamHI and NotI sites (pBS-Fluc).

    Article Title: Corticotropin-releasing factor and its receptors in the brain of rats with insulin and corticosterone deficits.
    Article Snippet: The CRF1R cRNA probe was generated from a 1$3 kb PstI–PstI fragment of the rat prCRF PP1.3-BS cDNA (Dr Vale W, Peptide Biology Laboratory, The Salk Institute) subcloned into a pBluescript II-SK vector (Stratagene) and linearized with BamHI and HindIII to generate antisense and sense probes respectively.

    Article Title: Complex Seizure Disorder Caused by Brunol4 Deficiency in Mice
    Article Snippet: The StuI-digested fragment of ∼3 kb was cloned into the pBluescript II-SK vector (Stratagene, http://www.stratagene.com ).

    Article Title: A single postnatal injection of oxytocin rescues the lethal feeding behaviour in mouse newborns deficient for the imprinted Magel2 gene.
    Article Snippet: The 2.651 kb XhoI and 3.062 kb NotI fragments localized, respectively, 372 and 3062 bp 5′ and 3′ to the transcriptional initiation site of the NT-039428.7 Magel2 mRNA (an intronless gene) were inserted on each side of a LoxP-pgk1-hygromycin-LoxP cassette subcloned into a pBluescript II-SK vector (Stratagene, Agilent, France).

    Article Title: The Polypyrimidine Tract-binding Protein Is Required for Efficient Dengue Virus Propagation and Associates with the Viral Replication Machinery
    Article Snippet: The firefly luciferase gene was amplified from the pGL3-Basic vector (Promega) using the primers Fluc Bam F and Fluc Not R and cloned into the pBluescript II-SK vector (Stratagene) at the BamHI and NotI sites (pBS-Fluc).

    Plasmid Preparation:

    Article Title: The Polypyrimidine Tract-binding Protein Is Required for Efficient Dengue Virus Propagation and Associates with the Viral Replication Machinery
    Article Snippet: The firefly luciferase genewas amplified from the pGL3Basic vector (Promega) using the primers FlucBamF and FlucNotR and cloned into the pBluescript II-SK vector (Stratagene) at the BamHI and NotI sites (pBS-Fluc).

    Article Title: Corticotropin-releasing factor and its receptors in the brain of rats with insulin and corticosterone deficits.
    Article Snippet: The CRF1R cRNA probe was generated from a 1$3 kb PstI–PstI fragment of the rat prCRF PP1.3-BS cDNA (Dr Vale W, Peptide Biology Laboratory, The Salk Institute) subcloned into a pBluescript II-SK vector (Stratagene) and linearized with BamHI and HindIII to generate antisense and sense probes respectively.

    Article Title: Complex Seizure Disorder Caused by Brunol4 Deficiency in Mice
    Article Snippet: The StuI-digested fragment of ∼3 kb was cloned into the pBluescript II-SK vector (Stratagene, http://www.stratagene.com ).

    Article Title: A single postnatal injection of oxytocin rescues the lethal feeding behaviour in mouse newborns deficient for the imprinted Magel2 gene.
    Article Snippet: The 2.651 kb XhoI and 3.062 kb NotI fragments localized, respectively, 372 and 3062 bp 5′ and 3′ to the transcriptional initiation site of the NT-039428.7 Magel2 mRNA (an intronless gene) were inserted on each side of a LoxP-pgk1-hygromycin-LoxP cassette subcloned into a pBluescript II-SK vector (Stratagene, Agilent, France).

    Article Title: The Polypyrimidine Tract-binding Protein Is Required for Efficient Dengue Virus Propagation and Associates with the Viral Replication Machinery
    Article Snippet: The firefly luciferase gene was amplified from the pGL3-Basic vector (Promega) using the primers Fluc Bam F and Fluc Not R and cloned into the pBluescript II-SK vector (Stratagene) at the BamHI and NotI sites (pBS-Fluc).

    Clone Assay:

    Article Title: The Polypyrimidine Tract-binding Protein Is Required for Efficient Dengue Virus Propagation and Associates with the Viral Replication Machinery
    Article Snippet: The firefly luciferase genewas amplified from the pGL3Basic vector (Promega) using the primers FlucBamF and FlucNotR and cloned into the pBluescript II-SK vector (Stratagene) at the BamHI and NotI sites (pBS-Fluc).

    Article Title: Corticotropin-releasing factor and its receptors in the brain of rats with insulin and corticosterone deficits.
    Article Snippet: The CRF1R cRNA probe was generated from a 1$3 kb PstI–PstI fragment of the rat prCRF PP1.3-BS cDNA (Dr Vale W, Peptide Biology Laboratory, The Salk Institute) subcloned into a pBluescript II-SK vector (Stratagene) and linearized with BamHI and HindIII to generate antisense and sense probes respectively.

    Article Title: Complex Seizure Disorder Caused by Brunol4 Deficiency in Mice
    Article Snippet: The StuI-digested fragment of ∼3 kb was cloned into the pBluescript II-SK vector (Stratagene, http://www.stratagene.com ).

    Article Title: A single postnatal injection of oxytocin rescues the lethal feeding behaviour in mouse newborns deficient for the imprinted Magel2 gene.
    Article Snippet: The 2.651 kb XhoI and 3.062 kb NotI fragments localized, respectively, 372 and 3062 bp 5′ and 3′ to the transcriptional initiation site of the NT-039428.7 Magel2 mRNA (an intronless gene) were inserted on each side of a LoxP-pgk1-hygromycin-LoxP cassette subcloned into a pBluescript II-SK vector (Stratagene, Agilent, France).

    Article Title: The Polypyrimidine Tract-binding Protein Is Required for Efficient Dengue Virus Propagation and Associates with the Viral Replication Machinery
    Article Snippet: The firefly luciferase gene was amplified from the pGL3-Basic vector (Promega) using the primers Fluc Bam F and Fluc Not R and cloned into the pBluescript II-SK vector (Stratagene) at the BamHI and NotI sites (pBS-Fluc).

    Generated:

    Article Title: The Polypyrimidine Tract-binding Protein Is Required for Efficient Dengue Virus Propagation and Associates with the Viral Replication Machinery
    Article Snippet: The firefly luciferase genewas amplified from the pGL3Basic vector (Promega) using the primers FlucBamF and FlucNotR and cloned into the pBluescript II-SK vector (Stratagene) at the BamHI and NotI sites (pBS-Fluc).

    Article Title: Corticotropin-releasing factor and its receptors in the brain of rats with insulin and corticosterone deficits.
    Article Snippet: The CRF1R cRNA probe was generated from a 1$3 kb PstI–PstI fragment of the rat prCRF PP1.3-BS cDNA (Dr Vale W, Peptide Biology Laboratory, The Salk Institute) subcloned into a pBluescript II-SK vector (Stratagene) and linearized with BamHI and HindIII to generate antisense and sense probes respectively.

    Article Title: Complex Seizure Disorder Caused by Brunol4 Deficiency in Mice
    Article Snippet: The StuI-digested fragment of ∼3 kb was cloned into the pBluescript II-SK vector (Stratagene, http://www.stratagene.com ).

    Article Title: A single postnatal injection of oxytocin rescues the lethal feeding behaviour in mouse newborns deficient for the imprinted Magel2 gene.
    Article Snippet: The 2.651 kb XhoI and 3.062 kb NotI fragments localized, respectively, 372 and 3062 bp 5′ and 3′ to the transcriptional initiation site of the NT-039428.7 Magel2 mRNA (an intronless gene) were inserted on each side of a LoxP-pgk1-hygromycin-LoxP cassette subcloned into a pBluescript II-SK vector (Stratagene, Agilent, France).

    Article Title: The Polypyrimidine Tract-binding Protein Is Required for Efficient Dengue Virus Propagation and Associates with the Viral Replication Machinery
    Article Snippet: The firefly luciferase gene was amplified from the pGL3-Basic vector (Promega) using the primers Fluc Bam F and Fluc Not R and cloned into the pBluescript II-SK vector (Stratagene) at the BamHI and NotI sites (pBS-Fluc).



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